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UniFrac provides a suite of tools for the comparison of microbial communities using phylogenetic information. It takes as input a single phylogenetic tree that contains sequences derived from at least two different environmental samples and a file describing which sequences came from which sample. For sample data click here. For citation, click here.

Both the UniFrac distance metric and the P test can be used to make comparisons. Both of these techniques bypass the need to choose operational taxonomic units (OTUs) based on sequence divergence prior to analysis.

UniFrac allows you to:

  • Determine if the environments in the input phylogenetic tree have significantly different microbial communities.
  • Determine if community differences are concentrated within particular lineages of the phylogenetic tree.
  • Cluster environments to determine whether there are environmental factors (such as temperature or salinity) that group communities together.
  • Determine whether the environments were sampled sufficiently to support cluster nodes.
  • Visualize the differences between environments graphically.

Please enter your email and password to continue. After you register you will be able to analyze up to 5000 sequences, up to 50 unique environments, and perform significance test based on up to 100 tree permutations. If you do not wish to register, you can analyze up to 100 sequences, up to 10 unique environments, and perform significance test based on up to 10 tree permutations using the guest account. The guest account email is guest and the password is guest.

We have released a new web application called "Fast Unifrac," that performs analyses faster, has enhanced visualization functionality, and higher default limits (100,000 unique sequences, 200 samples, and 1000 tree permutations). This is available at http://bmf2.colorado.edu/fastunifrac.

Notice: Knight Lab web applications will be unavailable June 28, 2011 for essential hardware maintenance. We apologize for any inconvenience

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